Serological evidence of human infection by bats orthoreovirus in Singapore

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005-2013. After an initial screen with lucferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific anitbodies against PRVin both assays. Our findings confirm that PRV spillover into human populations in relatively common in this region of the world.